Zcchc17^tm3Pou
Targeted Allele Detail

Summary

• Symbol: Zcchc17^tm3Pou
• Name: zinc finger, CCHC domain containing 17; targeted mutation 3, Pin Ouyang
• MGI ID: MGI:6147770
• Synonyms: deltaE2 + E4
• Gene: Zcchc17 Location: Chr4:130209176-130253736 bp, - strand Genetic Position: Chr4, 63.43 cM
• Alliance: Zcchc17^tm3Pou page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233160
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Insertion
• Mutation details: ES cells harboring the Zcchc17^tm1Pou (which contains a PGK-beta-geo (beta-galactosidase-neomycin fusion gene) selection cassette inserted into exon 4) allele were targeted to insert a PGK-hygromycin cassette into exon 2. Both the ATGs in exon 2 (long isoform) and exon 4 (short isoform) are disrupted. RT-PCR indicates enhanced mRNA expression of a miniform spanning exons 1, 6, and 7 (345 bp), which can produce a peptide from an alternative start codon in exon 7. (J:233160)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zcchc17 Mutation: 25 strains or lines available

References

• Original: J:233160 Lin YM, et al., Target disruption of ribosomal protein pNO40 accelerates aging and impairs osteogenic differentiation of mesenchymal stem cells. Biochem Biophys Res Commun. 2016 Jan 22;469(4):903-10
• All: 1 reference(s)