Ano5^tm1.2Naga
Targeted Allele Detail

Summary

• Symbol: Ano5^tm1.2Naga
• Name: anoctamin 5; targeted mutation 1.2, Shigekazu Nagata
• MGI ID: MGI:6148084
• Synonyms: deleted allele, TMEM16E-knockout
• Gene: Ano5 Location: Chr7:51160777-51248457 bp, + strand Genetic Position: Chr7, 32.76 cM
• Alliance: Ano5^tm1.2Naga page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236134
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A 1.6-kb DNA fragment containing exon 2 was flanked with loxP sites, with an frt-flanked neomycin resistance cassette inserted downstream of exon 2. The neomycin selection cassette was removed by crossing to Flp mice. Cre-mediated recombination resulted in the deletion of exon 2 in the germline. (J:236134)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ano5 Mutation: 67 strains or lines available

References

• Original: J:236134 Gyobu S, et al., A Role of TMEM16E Carrying a Scrambling Domain in Sperm Motility. Mol Cell Biol. 2015 Dec 14;36(4):645-59
• All: 1 reference(s)