Kras^tm1.1Khai
Targeted Allele Detail

Summary

• Symbol: Kras^tm1.1Khai
• Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 1.1, Kevin Haigis
• MGI ID: MGI:6149500
• Synonyms: Kras^LSL-A146T
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm1.1Khai page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276349
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: The targeting vector is designed to insert a loxP site flanked STOP cassette into intron 1. Exon 4 was modified to change alanine codon 146 to threonine (p.A146T), a mutation commonly found in patients with cancers of the gastrointestinal tract and blood. An FRT site flanked neomycin resistance gene cassette is placed into intron 4. Flp-mediated recombination removed the neo cassette. (J:276349)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Kras^tm1.1Khai

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 84 strains or lines available

References

• Original: J:276349 Poulin EJ, et al., Tissue-Specific Oncogenic Activity of KRAS(A146T). Cancer Discov. 2019 Jun;9(6):738-755
• All: 1 reference(s)