Zswim6tm1.2Itl
Targeted Allele Detail
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Symbol: |
Zswim6tm1.2Itl |
Name: |
zinc finger SWIM-type containing 6; targeted mutation 1.2, inGenious Targeting Laboratory |
MGI ID: |
MGI:6149981 |
Gene: |
Zswim6 Location: Chr13:107861152-108026598 bp, - strand Genetic Position: Chr13, 59.39 cM
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Alliance: |
Zswim6tm1.2Itl page
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Germline Transmission: |
Earliest citation of germline transmission:
J:259070
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Parent Cell Line: |
iTL BA1 (ES Cell)
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Strain of Origin: |
(C57BL/6NTac x 129S6/SvEvTac)F1
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Intragenic deletion
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Mutation details: A FRT-LoxP-Neomycin-FRT-LoxP selection cassette was inserted downstream of exon 4 and the third single LoxP site was inserted upstream of exon 4. The region flanked by the second and third LoxP sites is approximately 569 bp. Exon 4 encodes amino acids 337-386 and deletion of this sequence results in the creation of a premature stop codon. The neomycin selection cassette was removed via FLP-mediated recombination, leaving loxP sites flanking exon 4. Exon 4 was susequently removed via Cre-mediated recombination. In situ hybridization and RT-PCR analysis confirmed that this allele lacks expression of exon 4. qPCR analysis revealed that the medial ganglionic eminence of homozygous mutants expresses transcript downstream of the deleted region at ~35% of control levels at E13.5.
(J:259070)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Zswim6 Mutation: |
61 strains or lines available
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Original: |
J:259070 Tischfield DJ, et al., Loss of the neurodevelopmental gene Zswim6 alters striatal morphology and motor regulation. Neurobiol Dis. 2017 Jul;103:174-183 |
All: |
1 reference(s) |
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