Sh3bp2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sh3bp2^{tm1c(KOMP)Wtsi}
• Name: SH3-domain binding protein 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6150886
• Synonyms: Sh3bp2^fl
• Gene: Sh3bp2 Location: Chr5:34683182-34720985 bp, + strand Genetic Position: Chr5, 17.89 cM
• Alliance: Sh3bp2^{tm1c(KOMP)Wtsi} page
• IMPC: Sh3bp2 gene page

Mutation origin

• Mutant Cell Line: EPD0129_3_C05
• Germline Transmission: Earliest citation of germline transmission: J:243612
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34713803 of Chromosome 5 upstream of the critical exons 6 and 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 6 and 7 at position 34714983. The critical exons 6 and 7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:243612)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Sh3bp2 Mutation: 35 strains or lines available

References

• Original: J:243612 Matsumoto Y, et al., RANKL coordinates multiple osteoclastogenic pathways by regulating expression of ubiquitin ligase RNF146. J Clin Invest. 2017 Apr 03;127(4):1303-1315
• All: 2 reference(s)