Vipr2^{em1.1(cre)Hze}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Vipr2^{em1.1(cre)Hze}
• Name: vasoactive intestinal peptide receptor 2; endonuclease-mediated mutation 1.1, Hongkui Zeng
• MGI ID: MGI:6150903
• Synonyms: Vipr2-IRES2-Cre-D, Vipr2-IRES2-Cre-Delta, Vipr2-IRES2-Cre-Deltaneo
• Gene: Vipr2 Location: Chr12:116041346-116109881 bp, + strand Genetic Position: Chr12, 62.59 cM, cytoband F2
• Alliance: Vipr2^{em1.1(cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260362
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Vipr2^{em1.1(cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Vipr2 (MGI:107166)

• Mutation details: Embryonic stem (ES) cells were used for CRISPR/Cas9 genome engineering. The donor vector sequences contained, from 5' to 3', a partial Vipr2 sequence spanning intron 12 up to and including the endogenous stop codon in exon 13, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette, a frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. In addition, several basepair mutations were introduced into the first 30 nucleotides of the Vipr2 3' UTR within exon 13 to protect the donor vector from CRISPR/Cas9 cleavage. Correctly targeted ES cells (Vipr2-IRES2-Cre-neo genotype) were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK/gb2-Neo-pA::frt5::RNA splice acceptor::3'hygro-pA) and replace it with the recombined AttB/AttP site (AttL). (J:260362)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Vipr2 (mouse) Summary of all recombinase alleles driven by Vipr2. Comparative matrix view of recombinase activities Alliance mouse Vipr2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vipr2 Mutation: 31 strains or lines available

References

• Original: J:260362 Daigle TL, et al., A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality. Cell. 2018 Jul 12;174(2):465-480.e22
• All: 18 reference(s)