Igs7^{tm134.1(tetO-COP4*H134R/EYFP)Hze}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm134.1(tetO-COP4*H134R/EYFP)Hze}
• Name: intergenic site 7; targeted mutation 134.1, Hongkui Zeng
• MGI ID: MGI:6151056
• Synonyms: Ai134, Ai134(TITL-ChR2-YFP)
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm134.1(tetO-COP4*H134R/EYFP)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260362
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The vector is designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a Tet response element/promoter, a floxed-STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the ChR2(H134R)-EYFP fusion sequence, a WPRE (to enhance mRNA stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence and replaced it with the recombined AttB/AttP site (AttL). (J:260362)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 70 strains or lines available

References

• Original: J:260362 Daigle TL, et al., A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality. Cell. 2018 Jul 12;174(2):465-480.e22
• All: 1 reference(s)