Lrit1^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Lrit1^{tm1b(EUCOMM)Hmgu}
• Name: leucine-rich repeat, immunoglobulin-like and transmembrane domains 1; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6151090
• Gene: Lrit1 Location: Chr14:36776787-36786903 bp, + strand Genetic Position: Chr14, 21.29 cM
• Alliance: Lrit1^{tm1b(EUCOMM)Hmgu} page
• IMPC: Lrit1 gene page

Mutation origin

• Mutant Cell Line: HEPD0832_7_G03
• Germline Transmission: Earliest citation of germline transmission: J:260354
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 36778762 of Chromosome 14 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 36779917. The critical exon 2 is thus flanked by loxP sites. Cre expression without flp expression created a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blot analysis cofirmed the absence of protein expression in retinas. (J:260354)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lrit1 Mutation: 38 strains or lines available

References

• Original: J:260354 Sarria I, et al., LRIT1 Modulates Adaptive Changes in Synaptic Communication of Cone Photoreceptors. Cell Rep. 2018 Mar 27;22(13):3562-3573
• All: 1 reference(s)