Coro1c^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Coro1c^{tm1d(KOMP)Wtsi}
• Name: coronin, actin binding protein 1C; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6151182
• Gene: Coro1c Location: Chr5:113980500-114046767 bp, - strand Genetic Position: Chr5, 55.64 cM, cytoband F
• Alliance: Coro1c^{tm1d(KOMP)Wtsi} page
• IMPC: Coro1c gene page

Mutation origin

• Mutant Cell Lines: EPD0343_2_B04, EPD0343_2_F09
• Germline Transmission: Earliest citation of germline transmission: J:260533
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 113988438 of Chromosome 5 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 5 at position 113989346. The critical exon 5 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:260533)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Coro1c Mutation: 69 strains or lines available

References

• Original: J:260533 Behrens J, et al., Coronin 1C-free primary mouse fibroblasts exhibit robust rearrangements in the orientation of actin filaments, microtubules and intermediate filaments. Eur J Cell Biol. 2016 Aug;95(8):239-51
• All: 2 reference(s)