Cabyr^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cabyr^em1Osb
• Name: calcium binding tyrosine phosphorylation regulated; endonuclease-mediated mutation 1, Osaka University
• MGI ID: MGI:6152666
• Synonyms: Cabyr⁺¹⁷, Cabyr^em1Maik, Cabyr-KO
• Gene: Cabyr Location: Chr18:12874141-12888203 bp, + strand Genetic Position: Chr18, 6.77 cM, cytoband A2
• Alliance: Cabyr^em1Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 2, which contains the ATG translation start codon and is shared amongst all known splice variants, was targeted using an sgRNA and CRISPR/Cas9. The resulting allele has a 17 bp insertion in exon 2, resulting in a reading frame shift and subsequent premature stop codon in exon 3. Immunoblots of extracts from spermatozoa demonstrate the lack of protein expression from this allele. (J:247126)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cabyr Mutation: 29 strains or lines available

References

• Original: J:247126 Young SA, et al., CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa. J Cell Sci. 2016 Dec 01;129(23):4379-4387
• All: 1 reference(s)