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Ncf2tm1b(EUCOMM)Wtsi
Targeted Allele Detail
Summary
Symbol: Ncf2tm1b(EUCOMM)Wtsi
Name: neutrophil cytosolic factor 2; targeted mutation 1b, Wellcome Trust Sanger Institute
MGI ID: MGI:6153187
Synonyms: Ncf2-
Gene: Ncf2  Location: Chr1:152675945-152712742 bp, + strand  Genetic Position: Chr1, 65.05 cM
Alliance: Ncf2tm1b(EUCOMM)Wtsi page
IMPC: Ncf2 gene page
Mutation
origin
Mutant Cell Line:  EPD0240_5_F04
Germline Transmission:  Earliest citation of germline transmission: J:260777
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette containing a FRT-En2 splice acceptor (SA)-IRES-lacZ-SV40 polyadenylation signal (pA)-loxP-human Beta-actin promoter-neoR-SV40 polyadenylation signal (pA)-FRT-loxP sequence was inserted upstream of exon 3 and an additional loxP site downstream of exon 3 (at position 152817361). Cre-mediated recombination removed the floxed neo cassette and exon 3. No gene product (protein) is detected by western blot analysis of neutrophil cell lysates from homozygotes. (J:260777)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ncf2 Mutation:  39 strains or lines available
References
Original:  J:260777 Jacob CO, et al., Haploinsufficiency of NADPH Oxidase Subunit Neutrophil Cytosolic Factor 2 Is Sufficient to Accelerate Full-Blown Lupus in NZM 2328 Mice. Arthritis Rheumatol. 2017 Aug;69(8):1647-1660
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory