Far1^{em1(IMPC)Wtsi}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Far1^{em1(IMPC)Wtsi}
• Name: fatty acyl CoA reductase 1; endonuclease-mediated mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:6153339
• Synonyms: Far1^-, Far1 KO
• Gene: Far1 Location: Chr7:113113041-113170718 bp, + strand Genetic Position: Chr7, 59.21 cM, cytoband F2
• Alliance: Far1^{em1(IMPC)Wtsi} page
• IMPC: Far1 gene page

Mutation origin

• Strain of Origin: C57BL/6N
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from IMPC was generated at the Welcome Trust Sanger Institute. Four sgRNAs were used to target the 5 and 3 intronic flanking regions thereby deleting in frame exon 5, which encodes for 50 amino acids. Sanger Sequencing confirmed the CRISPR/Cas9-mediated deletion of 475 bp including the deletion of the entire exon 5. Western blot analysis demonstrated absence of protein expression in testis extracts from homozygous mutant male mice. (J:237616, J:339470)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Far1 Mutation: 27 strains or lines available

References

• Original: J:237616 MGI and IMPC, MGI Curation of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). MGI Direct Data Submission. 2017-8
• All: 4 reference(s)