Hmces^em1Dfel
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hmces^em1Dfel
• Name: 5-hydroxymethylcytosine (hmC) binding, ES cell specific; endonuclease-mediated mutation 1, Douglas E Feldman
• MGI ID: MGI:6154268
• Synonyms: Srap1 delta388
• Gene: Hmces Location: Chr6:87890917-87913611 bp, + strand Genetic Position: Chr6, 39.13 cM
• Alliance: Hmces^em1Dfel page

Mutation origin

• Strain of Origin: (C57BL/6 x 129)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cas9 mRNA and single-guide RNA (sgRNA) targeting exon 2 was microinjected into the cytoplasm of zygote-stage embryos to induce deletions. Injected embryos were implanted in CD-1 foster mice and pups screened for deletions. Two lines were generated. This allele has a 388 bp deletion encompassing the translation initiation codon and catalytic invariant Cys2 codon. (J:254234)

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hmces Mutation: 19 strains or lines available

References

• Original: J:254234 Kweon SM, et al., Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease. Cell Rep. 2017 Oct 10;21(2):482-494
• All: 3 reference(s)