Tg(Thy1-jRGECO1a)GP8.5Kim
Transgene Detail

Summary

• Symbol: Tg(Thy1-jRGECO1a)GP8.5Kim
• Name: transgene insertion GP8.5, Douglas Kim
• MGI ID: MGI:6154284
• Transgene: Tg(Thy1-jRGECO1a)GP8.5Kim Location: unknown
• Alliance: Tg(Thy1-jRGECO1a)GP8.5Kim page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Transposon induced (Reporter)
• Mutation: Insertion
• Mutation details: The mouse Thy1 promoter drives expression of this transgene that includes jRGECO1a, nuclear export signal (NES) from protein kinase inhibitor alpha (PKIalpha), woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and bovine growth hormone (bGH) polyadenylation (polyA) sequence. jRGECO1a is a modified form of R-GECO. R-GECO is derived from mApple (a red fluorescent monomer which is substantially brighter than mCherry and displays complex photoswitching behavior) fused to calmodulin and the calmodulin binding region of chicken myosin light chain kinase (M13). The calmodulin region is modified with Q306D and M339F mutations. The M339F mutation accelerates kinetics, but does not affect response amplitude. For cortical imaging, founder line GP8.5 (Dana, 2018) has moderate labeling density of cells. The GP8.5 line provides moderate signal levels per cell across various cortical regions. The GP8.5 line also exhibits retinal expression, including the ganglion cell layer. (J:268005)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:268005 Dana H, et al., Thy1 transgenic mice expressing the red fluorescent calcium indicator jRGECO1a for neuronal population imaging in vivo. PLoS One. 2018;13(10):e0205444
• All: 1 reference(s)