Slc17a6^{tm1.1(flpo)Jnb}
Targeted Allele Detail

Summary

• Symbol: Slc17a6^{tm1.1(flpo)Jnb}
• Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6; targeted mutation 1.1, J Nicholas Betley
• MGI ID: MGI:6156365
• Synonyms: VGlut2-IRES-FlpO
• Gene: Slc17a6 Location: Chr7:51271754-51320867 bp, + strand Genetic Position: Chr7, 32.78 cM, cytoband B4
• Alliance: Slc17a6^{tm1.1(flpo)Jnb} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:261454
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Slc17a6^{tm1.1(flpo)Jnb} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc17a6 (MGI:2156052)

• Mutation details: The targeting vector containing a 8,572 bp genomic DNA fragment spanning exon 9-12 of mouse Slc17a6 (VGlut2) and a cassette of IRES-FlpO-loxP2272-ACE-Cre POII NeoR-loxp2272 inserted between stop codon TAA and 3' UTR was introduced into ES cells. Positive ES clones were expanded for generation of chimeric mice which have gone through self excised NeoR cassette. (J:261454)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc17a6 (mouse) Summary of all recombinase alleles driven by Slc17a6. Alliance mouse Slc17a6 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc17a6 Mutation: 60 strains or lines available

Notes

The targeting vector was electroporated into F1 hybrid of 129S6 x C57BL/6J ES cells derived by the Janelia Transgenic Facility.

References

• Original: J:261454 Alhadeff AL, et al., A Neural Circuit for the Suppression of Pain by a Competing Need State. Cell. 2018 Mar 22;173(1):140-152.e15
• All: 1 reference(s)