Atp8b1^em1(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Atp8b1^em1(IMPC)Tcp
• Name: ATPase, class I, type 8B, member 1; endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:6156424
• Gene: Atp8b1 Location: Chr18:64662050-64794342 bp, - strand Genetic Position: Chr18, 37.49 cM
• Alliance: Atp8b1^em1(IMPC)Tcp page
• IMPC: Atp8b1 gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project TCPR0945 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of AACTCCAAGTGGGTAGGGTC and AAAATCGAGTATCCACCACC targeting the 5' side and CTAGAAGTCTATGGCACATT and TGCATTGCTGCAGGTACATA targeting the 3' side of critical exons resulting in a 493-bp deletion Chr18:64577258 to 64577750 (GRCm38). (J:237616)

Expression

In Structures Affected by this Mutation:

15 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Atp8b1 Mutation: 78 strains or lines available

References

• Original: J:237616 MGI and IMPC, MGI Curation of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). MGI Direct Data Submission. 2017-8
• All: 3 reference(s)