Upf3b^em1(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Upf3b^em1(IMPC)Tcp
• Name: UPF3 regulator of nonsense transcripts homolog B (yeast); endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:6156436
• Gene: Upf3b Location: ChrX:36355331-36373975 bp, - strand Genetic Position: ChrX, 21.51 cM, cytoband A2
• Alliance: Upf3b^em1(IMPC)Tcp page
• IMPC: Upf3b gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project TCPR0565 was generated at The Centre for Phenogenomics by injecting Cas9 mRNA and four guide RNAs with spacer sequences of GGAGATCACTTAGTTGGCCT and ATGGCATTTCTTCTACTATA targeting the 5' side and TTATTCACTGCGAACTAATA and TATATCCCCTGAGGCTGTCA targeting the 3' side of exons ENSMUSE00001311515 and ENSMUSE00001291484. This resulted in a 2471-bp deletion of ChrX from 37099863 to 37102333_insTGT (GRCm38). (J:237616)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Upf3b Mutation: 13 strains or lines available

References

• Original: J:237616 MGI and IMPC, MGI Curation of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). MGI Direct Data Submission. 2017-8
• All: 3 reference(s)