Pramel1^em1(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pramel1^em1(IMPC)Tcp
• Name: PRAME like 1; endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:6156469
• Gene: Pramel1 Location: Chr4:143120998-143126730 bp, + strand Genetic Position: Chr4, 77.64 cM
• Alliance: Pramel1^em1(IMPC)Tcp page
• IMPC: Pramel1 gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project TCPR0984 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of GATCATAAAGGCGATGGTGC and GCAAGGCATCAAGCCCATAC targeting the 5' side and CCCGAATGGCCCACCACTGC targeting the 3' side of a critical region. This resulted in a 454-bp deletion Chr4:143396699 to 143397152; p.I82S*fs37 (GRCm38). (J:237616)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pramel1 Mutation: 21 strains or lines available

References

• Original: J:237616 MGI and IMPC, MGI Curation of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). MGI Direct Data Submission. 2017-8
• All: 3 reference(s)