Pikfyve^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Pikfyve^{tm1b(EUCOMM)Hmgu}
• Name: phosphoinositide kinase, FYVE type zinc finger containing; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6156635
• Synonyms: PIKfyve^flox
• Gene: Pikfyve Location: Chr1:65225842-65317854 bp, + strand Genetic Position: Chr1, 32.94 cM, cytoband C3
• Alliance: Pikfyve^{tm1b(EUCOMM)Hmgu} page
• IMPC: Pikfyve gene page

Mutation origin

• Mutant Cell Lines: HEPD0682_4_B04, HEPD0682_4_D04
• Germline Transmission: Earliest citation of germline transmission: J:244776
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 65235192 of Chromosome 1 upstream of the critical exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 65236231. The critical exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:244776)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pikfyve Mutation: 116 strains or lines available

Notes

Cell line HEPD0682_4_B04 and HEPD0682_4_D04 were successfully used to make chimeric mice. Germline transmission was accomplished from a mixed population. J:244776

References

• Original: J:244776 Kawasaki T, et al., Deletion of PIKfyve alters alveolar macrophage populations and exacerbates allergic inflammation in mice. EMBO J. 2017 Jun 14;36(12):1707-1718
• All: 1 reference(s)