Gt(ROSA)26Sor^{tm1(CAG-mNeonGreen/mRuby2)Mnz}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-mNeonGreen/mRuby2)Mnz}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Michel C Nussenzweig
• MGI ID: MGI:6156806
• Synonyms: Rosa26^LSL-INDIA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-mNeonGreen/mRuby2)Mnz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:261534
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A CAG promoter, loxP-flanked STOP/neomycin cassette in reverse transcriptional orientation, a FRET-based INDIcator of Apoptosis (INDIA) cassette, a single FRT site (remaining after Flp-expressing bacteria were used to excise an FRT-flanked IRES-EGFP cassette from the targeting vector), and polyA were introduced to intron 1. The INDIA reporter consists of an optimized fluorescence (Forster) resonance energy transfer (FRET) pair: N-terminally Myc-tagged mNeonGreen and mRuby2 linked by an 18 amino acid peptide containing an aCasp3-cleavage site (DEVDG). When aCasp3 (Casp3, caspase 3; a marker for apoptosis) cleaves the linker, mNeonGreen and mRuby2 are separated, resulting in FRET loss (FRET<->) and increased emission from mNeonGreen. Non-apoptotic cells retaining uncleaved mNeonGreen/mRuby2 (FRET<+>) demonstrate more yellow fluorescence indicating a lower ratio of mNeonGreen/FRET fluorescence emission. (J:261534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:261534 Mayer CT, et al., The microanatomic segregation of selection by apoptosis in the germinal center. Science. 2017 Oct 13;358(6360)
• All: 1 reference(s)