Fbxw7^tm1.1Iken
Targeted Allele Detail

Summary

• Symbol: Fbxw7^tm1.1Iken
• Name: F-box and WD-40 domain protein 7; targeted mutation 1.1, Tsuneo Ikenoue
• MGI ID: MGI:6157076
• Synonyms: Fbxw7^R468C
• Gene: Fbxw7 Location: Chr3:84722575-84886505 bp, + strand Genetic Position: Chr3, 37.7 cM, cytoband E3.3
• Alliance: Fbxw7^tm1.1Iken page

Developmental defects of the eye lids and oral cavity in Fbxw7^tm1.1Iken/Fbxw7⁺ mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258194
• Parent Cell Line: EGR-101 (ES Cell)
• Strain of Origin: C57BL/6NSlc

Mutation description

Allele Type: Targeted (Not Specified) Mutations: Insertion, Nucleotide substitutions Mutation details: Exons 6-10 were replaced with a construct containing exons 6 and 7, a loxP site, a minigene containing exons 8-11, a polyadenylation STOP sequence, a FRT-flanked neomycin resistance gene, a second loxP site, a mutated exon 8, and exons 9-10. The mutated exon 8 contains a C to T mutation at position 1402 and a G to T mutation at position 1404, resulting in an arginine to cysteine change at amino acid 468. The R468C mutation corresponds to the human R465C mutation, frequently detected in primary tumors. Cre-mediated recombination removed the floxed minigene and selection cassette, leaving a mutated exon 8. (J:258194)

Generation of the Fbxw7^tm1Iken and Fbxw7^tm1.1Iken alleles

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fbxw7 Mutation: 84 strains or lines available

References

• Original: J:258194 Ikenoue T, et al., Establishment and analysis of a novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation. Sci Rep. 2018 Jan 31;8(1):2021
• All: 1 reference(s)