Manf^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Manf^{tm1c(KOMP)Wtsi}
• Name: mesencephalic astrocyte-derived neurotrophic factor; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6157444
• Synonyms: Manf^fl, Manf^flox
• Gene: Manf Location: Chr9:106765952-106769137 bp, - strand Genetic Position: Chr9, 57.98 cM, cytoband F1
• Alliance: Manf^{tm1c(KOMP)Wtsi} page
• IMPC: Manf gene page

Mutation origin

• Mutant Cell Line: EPD0162_3_D06
• Germline Transmission: Earliest citation of germline transmission: J:211812
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 106767151 of Chromosome 9 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 106767840. A "conditional ready" (floxed) allele was created through flp-mediate recombination, leaving exon 3 floxed. (J:211812)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Manf Mutation: 12 strains or lines available

References

• Original: J:211812 Lindahl M, et al., MANF is indispensable for the proliferation and survival of pancreatic beta cells. Cell Rep. 2014 Apr 24;7(2):366-75
• All: 14 reference(s)