Klhl3^tm2.1Esoh
Targeted Allele Detail

Summary

• Symbol: Klhl3^tm2.1Esoh
• Name: kelch-like 3; targeted mutation 2.1, Eisei Sohara
• MGI ID: MGI:6157453
• Synonyms: KLHL3^-
• Gene: Klhl3 Location: Chr13:58148042-58261406 bp, - strand Genetic Position: Chr13, 30.92 cM
• Alliance: Klhl3^tm2.1Esoh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244278
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector was obtained from the KOMP repository at UC Davis and is composed of an FRT site followed by lacZ sequence and a loxP site. The first loxP site is followed by neomycin resistance gene, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3. The critical exon 3 is thus flanked by loxP sites. Cre-mediated recombination removed the neomycin gene and exon 3, resulting in a reporter knockout mouse. Western blot confirmed absence of protein in the brain and kidney. (J:244278)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klhl3 Mutation: 43 strains or lines available

References

• Original: J:244278 Sasaki E, et al., KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pathophysiology of Pseudohypoaldosteronism Type II Caused by Mutant KLHL3. Mol Cell Biol. 2017 Apr 01;37(7)
• All: 1 reference(s)