Notch2^tm2.2Ecan
Targeted Allele Detail

Summary

• Symbol: Notch2^tm2.2Ecan
• Name: notch 2; targeted mutation 2.2, Ernesto Canalis
• MGI ID: MGI:6157623
• Synonyms: Notch2^deltaPEST
• Gene: Notch2 Location: Chr3:97920854-98057683 bp, + strand Genetic Position: Chr3, 42.42 cM
• Alliance: Notch2^tm2.2Ecan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:246017
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A conditional by inversion (COIN) intron consisting of a lox66_SA-HA tag-IRES-Egfp-rabbit beta-globin polyA_lox71 was inserted in the antisense orientation after AAG codon encoding lysine 2384 in exon 34 via homologous recombination. The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition an FRT flanked UBp-neomycin selection cassette was inserted after the COIN intron. Flp-mediated recombination removed the neomycin cassette. Cre-mediated recombination inverted the inserted sequence resulting in the expression of an HA-tagged truncated protein lacking the PEST domain and Egfp. (J:246017)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Notch2 Mutation: 99 strains or lines available

References

• Original: J:246017 Zanotti S, et al., Sustained Notch2 signaling in osteoblasts, but not in osteoclasts, is linked to osteopenia in a mouse model of Hajdu-Cheney syndrome. J Biol Chem. 2017 Jul 21;292(29):12232-12244
• All: 2 reference(s)