Brpf3^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Brpf3^{tm1b(EUCOMM)Wtsi}
• Name: bromodomain and PHD finger containing, 3; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6159126
• Synonyms: Brpf3^DELTA
• Gene: Brpf3 Location: Chr17:29020088-29057763 bp, + strand Genetic Position: Chr17, 15.03 cM
• Alliance: Brpf3^{tm1b(EUCOMM)Wtsi} page
• IMPC: Brpf3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231042
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_gt0 cassette was inserted at position 29035613 of Chromosome 17 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site flanked lacZ reporter/neomycin resistance gene cassette followed by a loxP site. An additional loxP site is inserted downstream of exon 7 at position 29037893. Exons are thus flanked by loxP sites. Exons 5-7 were deleted through subsequent cre-mediated recombination, leaving a reporter-null allele. RT-PCR and immunoblotting experiments indicate that a truncated peptide is produced from this allele. (J:231042)

Expression

In Mice Carrying this Mutation:

14 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brpf3 Mutation: 71 strains or lines available

References

• Original: J:231042 Yan K, et al., The Chromatin Regulator BRPF3 Preferentially Activates the HBO1 Acetyltransferase but Is Dispensable for Mouse Development and Survival. J Biol Chem. 2016 Feb 5;291(6):2647-63
• All: 1 reference(s)