Nae1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nae1^{tm1c(EUCOMM)Wtsi}
• Name: NEDD8 activating enzyme E1 subunit 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6159278
• Gene: Nae1 Location: Chr8:105237660-105261269 bp, - strand Genetic Position: Chr8, 53.04 cM
• Alliance: Nae1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Nae1 gene page

Mutation origin

• Mutant Cell Line: EPD0441_1_C08
• Germline Transmission: Earliest citation of germline transmission: J:261328
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: Flp-mediated excision of the parental Nae1^{tm1a(EUCOMM)Wtsi} allele resulted in the removal of the promoter-driven neomycin selection cassette and lacZ cassette leaving exon 4 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:261328)

Expression

In Mice Carrying this Mutation:	6 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nae1 Mutation: 33 strains or lines available

References

• Original: J:261328 Zou J, et al., Neddylation mediates ventricular chamber maturation through repression of Hippo signaling. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4101-E4110
• All: 8 reference(s)