Cdk1^tm3Kald
Targeted Allele Detail

Summary

• Symbol: Cdk1^tm3Kald
• Name: cyclin dependent kinase 1; targeted mutation 3, Philipp Kaldis
• MGI ID: MGI:6160101
• Synonyms: Cdk1^SAF, Cdk1^StopAF
• Gene: Cdk1 Location: Chr10:69170976-69188742 bp, - strand Genetic Position: Chr10, 36.07 cM
• Alliance: Cdk1^tm3Kald page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262094
• Parent Cell Line: S1B6 (ES Cell)
• Strain of Origin: 129S1/SvImJ or C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A splice acceptor-STOP/neomycin selection cassette flanked by loxP sites was introduced on the 5 end of exon 3, which was modified to introduce point mutations that generate a threonine to alanine substitution at amino acid 14 (T14A) and a tyrosine to phenylalanine substitution at amino acid 15 (Y15F). A14 and F15 cannot be phosphorylated and thus this mutant becomes non-inhibitable by phosphorylation. Cre-mediated recombination is necessary to remove the loxP-flanked STOP cassette and get expression of the mutant protein. (J:262094)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk1 Mutation: 21 strains or lines available

References

• Original: J:262094 Adhikari D, et al., Inhibitory phosphorylation of Cdk1 mediates prolonged prophase I arrest in female germ cells and is essential for female reproductive lifespan. Cell Res. 2016 Nov;26(11):1212-1225
• All: 3 reference(s)