Cdk1tm3Kald
Targeted Allele Detail
|
Symbol: |
Cdk1tm3Kald |
Name: |
cyclin dependent kinase 1; targeted mutation 3, Philipp Kaldis |
MGI ID: |
MGI:6160101 |
Synonyms: |
Cdk1SAF, Cdk1StopAF |
Gene: |
Cdk1 Location: Chr10:69170976-69188742 bp, - strand Genetic Position: Chr10, 36.07 cM
|
Alliance: |
Cdk1tm3Kald page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:262094
|
Parent Cell Line: |
S1B6 (ES Cell)
|
Strain of Origin: |
129S1/SvImJ or C57BL/6J
|
|
Allele Type: |
|
Targeted (Conditional ready) |
Mutations: |
|
Insertion, Nucleotide substitutions
|
|
|
Mutation details: A splice acceptor-STOP/neomycin selection cassette flanked by loxP sites was introduced on the 5 end of exon 3, which was modified to introduce point mutations that generate a threonine to alanine substitution at amino acid 14 (T14A) and a tyrosine to phenylalanine substitution at amino acid 15 (Y15F). A14 and F15 cannot be phosphorylated and thus this mutant becomes non-inhibitable by phosphorylation. Cre-mediated recombination is necessary to remove the loxP-flanked STOP cassette and get expression of the mutant protein.
(J:262094)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Cdk1 Mutation: |
20 strains or lines available
|
|
Original: |
J:262094 Adhikari D, et al., Inhibitory phosphorylation of Cdk1 mediates prolonged prophase I arrest in female germ cells and is essential for female reproductive lifespan. Cell Res. 2016 Nov;26(11):1212-1225 |
All: |
3 reference(s) |
|