Gt(ROSA)26Sor^{tm1.1(Cyp1a1,Thy1)Stck}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(Cyp1a1,Thy1)Stck}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Brigitta Stockinger
• MGI ID: MGI:6161764
• Synonyms: R26^LSL-Cyp1a1
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(Cyp1a1,Thy1)Stck} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244974
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A minigene composed of the coding sequences of mouse Cyp1a1 and rat Thy1 connected by a 2A sequence followed by the Woodchuck hepatitis virus derived regulatory element and a bovine growth hormone polyadenylation site was inserted into the locus. In addition a cassette containing an FRT-flanked neomycin-resistance gene and loxP-flanked transcriptional stop elements were inserted upstream of the minigene. Flp-mediated recombination removed the neomycin resistance gene, leaving the floxed STOP cassette. This creates a conditional-ready allele that will only be expressed after cre-mediated deletion of the STOP cassette. (J:244974)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 958 strains or lines available

References

• Original: J:244974 Schiering C, et al., Feedback control of AHR signalling regulates intestinal immunity. Nature. 2017 Feb 09;542(7640):242-245
• All: 2 reference(s)