Tg(Tie2-Gja5*,-EGFP)#Dach
Transgene Detail

Summary

• Symbol: Tg(Tie2-Gja5*,-EGFP)#Dach
• Name: transgene insertion, Daniel J Chaston
• MGI ID: MGI:6162535
• Synonyms: Cx40T202STg
• Transgene: Tg(Tie2-Gja5*,-EGFP)#Dach Location: unknown
• Alliance: Tg(Tie2-Gja5*,-EGFP)#Dach page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(Tie2-Gja5*,-EGFP)#Dach expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gja5 (MGI:95716)	A>T substitution giving rise to Thr202Ser change in encoded peptide

• Mutation details: A transgene construct containing the following elements, in order, randomly inserted into the genome: the Tie2 promoter, a 1.3kb BamHI/NaeI Cx40 DNA segement, an IRES, the EGFP fluorescent reporter gene, a human growth hormone polyadenylation signal, and a Tie2 enhancer. The coding sequence of the inserted gene has an engineered A>T substitution at coding nucleotide 604 causing threonine codon 202 to change to a serine codon (p.Thr202Ser). This mutation is located in a conserved functional peptide sequence. (J:218811)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:218811 Chaston DJ, et al., Polymorphism in endothelial connexin40 enhances sensitivity to intraluminal pressure and increases arterial stiffness. Arterioscler Thromb Vasc Biol. 2013 May;33(5):962-70
• All: 2 reference(s)