Spata20^{Tn(pb-Act-RFP)1.1Zhu}
Transposon induced Allele Detail

Summary

• Symbol: Spata20^{Tn(pb-Act-RFP)1.1Zhu}
• Name: spermatogenesis associated 20; transposon insertion 1.1, Yuan Zhuang
• MGI ID: MGI:6163619
• Synonyms: Ssp411^PB
• Gene: Spata20 Location: Chr11:94369730-94376136 bp, - strand Genetic Position: Chr11, 58.9 cM
• Alliance: Spata20^{Tn(pb-Act-RFP)1.1Zhu} page

Mutation origin

• Strain of Origin: FVB/NJ

Mutation description

• Allele Type: Transposon induced (Null/knockout)
• Mutation: Transposon insertion
• Mutation details: The transposon containing an unspecified hybrid actin promoter driving expression of red fluorescent protein (RFP) flanked by piggyBac-specific left and right terminal sequences was co-injected with a piggyBac-containing transgene to mobilize the transposon. Line 1 contains a transposon inserted in the 13th intron of the gene. A RT PCR assay indicated that the encoded mRNA was truncated and degraded. Western blot analysis did not detect the encoded protein in the testis of homozygous mutant mice. (J:123680, J:262160)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spata20 Mutation: 32 strains or lines available

References

• Original: J:262160 Liu M, et al., Disruption of Ssp411 causes impaired sperm head formation and male sterility in mice. Biochim Biophys Acta. 2018 Mar;1862(3):660-668
• All: 2 reference(s)