Gt(ROSA)26Sor^{em1(ptxA)Btar}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(ptxA)Btar}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Basile Tarchini
• MGI ID: MGI:6163665
• Synonyms: mycPTXa
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(ptxA)Btar} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: Cas9 was used to mediate the insertion, into the Xba site between exons 1 and 2, of a 4kb SA-(loxP-stop-loxP)-mycPTXa cassette, resulting in this knockin allele in which exposure to Cre recombinase will excise the loxP-flaked stop cassette allowing expression of the catalytic subunit of Pertussis toxin. There is a single myc tag in-frame N-terminal to Pertussis toxin. (J:236519)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:236519 Tarchini B, et al., A link between planar polarity and staircase-like bundle architecture in hair cells. Development. 2016 Nov 1;143(21):3926-3932
• All: 8 reference(s)