Rnf128^tm1.2Cmse
Targeted Allele Detail

Summary

• Symbol: Rnf128^tm1.2Cmse
• Name: ring finger protein 128; targeted mutation 1.2, Christine M Seroogy
• MGI ID: MGI:6164139
• Gene: Rnf128 Location: ChrX:138464089-138573894 bp, + strand Genetic Position: ChrX, 61.35 cM
• Alliance: Rnf128^tm1.2Cmse page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:245025
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 was flanked by loxP sites and a FRT-flanked PGK-neomycin cassette was inserted downstream of the floxed exon 2. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination deleted exon 2. Deletion of exon 2 (bp 485-732) results in a premature stop codon at amino acid 168. (J:245025)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Rnf128^tm1.2Cmse/Rnf128^tm1.2Cmse	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms
hematopoietic system	√
abnormal macrophage physiology	√
homeostasis/metabolism	N
homeostasis/metabolism phenotype	N
immune system	√
abnormal macrophage physiology	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf128 Mutation: 10 strains or lines available

References

• Original: J:245025 Waqas SFH, et al., Neuropeptide FF increases M2 activation and self-renewal of adipose tissue macrophages. J Clin Invest. 2017 Jun 30;127(7):2842-2854
• All: 1 reference(s)