Dcaf17^tm1.1Assi
Targeted Allele Detail

Summary

• Symbol: Dcaf17^tm1.1Assi
• Name: DDB1 and CUL4 associated factor 17; targeted mutation 1.1, Abdullah Assiri
• MGI ID: MGI:6164306
• Gene: Dcaf17 Location: Chr2:70885672-70929486 bp, + strand Genetic Position: Chr2, 42.38 cM
• Alliance: Dcaf17^tm1.1Assi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262620
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A FRT/loxP flanked neomycin cassette was inserted upstream of exon 4 in intron 3-4 in an opposite direction in relation to the target gene. A single loxP site was inserted downstream of exon 4. Cre-mediated recombination removed both the selection cassette and exon 4. cDNA sequencing revealed that excision of exon 4 leads to a frameshift mutation with concomitant premature stop codon after 59 residues. (J:262620)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dcaf17 Mutation: 33 strains or lines available

References

• Original: J:262620 Ali A, et al., Deletion of DDB1- and CUL4- associated factor-17 (Dcaf17) gene causes spermatogenesis defects and male infertility in mice. Sci Rep. 2018 Jun 15;8(1):9202
• All: 2 reference(s)