Gas2^{tm1(GFP/cre/ERT2)Pzg}
Targeted Allele Detail

Summary

• Symbol: Gas2^{tm1(GFP/cre/ERT2)Pzg}
• Name: growth arrest specific 2; targeted mutation 1, Pumin Zhang
• MGI ID: MGI:6187669
• Gene: Gas2 Location: Chr7:51511763-51644723 bp, + strand Genetic Position: Chr7, 32.87 cM
• Alliance: Gas2^{tm1(GFP/cre/ERT2)Pzg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251841
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Gas2^{tm1(GFP/cre/ERT2)Pzg} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gas2 (MGI:95657)

• Mutation details: A GFP-Cre/ERT2 cassette and FRT-flanked Kanamycin/neomycin selection cassette were inserted into the first coding exon. RT-PCR and Western blot analysis confirmed absence of mRNA and protein, respectively, in homozygotes. (J:251841)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gas2 (mouse) Summary of all recombinase alleles driven by Gas2. Alliance mouse Gas2 gene page

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gas2 Mutation: 18 strains or lines available

References

• Original: J:251841 York JP, et al., Growth Arrest Specific 2 (GAS2) is a Critical Mediator of Germ Cell Cyst Breakdown and Folliculogenesis in Mice. Sci Rep. 2016 Oct 13;6:34956
• All: 1 reference(s)