Etfbkmt^em1Yshk
Endonuclease-mediated Allele Detail

Summary

• Symbol: Etfbkmt^em1Yshk
• Name: electron transfer flavoprotein beta subunit lysine methyltransferase; endonuclease-mediated mutation 1, Yoichi Shinkai
• MGI ID: MGI:6188078
• Synonyms: Mettl20 KO
• Gene: Etfbkmt Location: Chr6:149042980-149052669 bp, + strand Genetic Position: Chr6, 78.8 cM
• Alliance: Etfbkmt^em1Yshk page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 mutagenesis generated a 17 bp deletion in exon 4 which encodes the methyltransferase domain. Western blot analysis showed that protein expression levels were undetectable in all tissues tested in homozygous mutant mice. No tri-methylation of electron transfer flavoprotein beta subunit (ETFB) was detected in liver samples from homozygous mutant mice. (J:260200)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Etfbkmt Mutation: 31 strains or lines available

References

• Original: J:260200 Shimazu T, et al., Role of METTL20 in regulating beta-oxidation and heat production in mice under fasting or ketogenic conditions. Sci Rep. 2018 Jan 19;8(1):1179
• All: 1 reference(s)