Scyl3^tm1.1Spel
Targeted Allele Detail

Summary

• Symbol: Scyl3^tm1.1Spel
• Name: SCY1-like 3 (S. cerevisiae); targeted mutation 1.1, Stephane Pelletier
• MGI ID: MGI:6188894
• Gene: Scyl3 Location: Chr1:163756669-163782695 bp, + strand Genetic Position: Chr1, 71.23 cM
• Alliance: Scyl3^tm1.1Spel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262629
• Parent Cell Line: CMTI-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 5. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 6. Cre-mediated removed exons 5 and 6 along with the selection cassette. The absence of protein expression was confirmed by western blot analysis on mouse embryonic fibroblasts. (J:262629)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scyl3 Mutation: 32 strains or lines available

References

• Original: J:262629 Kuliyev E, et al., Overlapping Role of SCYL1 and SCYL3 in Maintaining Motor Neuron Viability. J Neurosci. 2018 Feb 7
• All: 1 reference(s)