Sned1^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sned1^{tm1d(KOMP)Wtsi}
• Name: sushi, nidogen and EGF-like domains 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6189216
• Gene: Sned1 Location: Chr1:93163563-93228787 bp, + strand Genetic Position: Chr1, 47.21 cM
• Alliance: Sned1^{tm1d(KOMP)Wtsi} page
• IMPC: Sned1 gene page

Mutation origin

• Mutant Cell Line: EPD0300_5_E02
• Germline Transmission: Earliest citation of germline transmission: J:295931
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion of gene trap vector, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 93186212 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 93187769. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:295931)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sned1 Mutation: 92 strains or lines available

References

• Original: J:295931 Barque A, et al., Knockout of the gene encoding the extracellular matrix protein SNED1 results in early neonatal lethality and craniofacial malformations. Dev Dyn. 2020 Oct 4
• All: 1 reference(s)