Klhl14^tm1.1Jyw
Targeted Allele Detail

Summary

• Symbol: Klhl14^tm1.1Jyw
• Name: kelch-like 14; targeted mutation 1.1, Ji-Yang Wang
• MGI ID: MGI:6191749
• Gene: Klhl14 Location: Chr18:21683434-21787775 bp, - strand Genetic Position: Chr18, 11.96 cM
• Alliance: Klhl14^tm1.1Jyw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:263511
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 21757262 of Chromosome 18 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 21759352. Cre-mediated recombination removed exon 3 and the selection cassette leaving behind the reporter cassette. (J:263511)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klhl14 Mutation: 32 strains or lines available

References

• Original: J:263511 Li S, et al., Kelch-like protein 14 promotes B-1a but suppresses B-1b cell development. Int Immunol. 2018 Jun 26;30(7):311-318
• All: 1 reference(s)