Rem2^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Rem2^{tm1c(EUCOMM)Hmgu}
• Name: rad and gem related GTP binding protein 2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6192081
• Synonyms: Rem2^flx
• Gene: Rem2 Location: Chr14:54713676-54717889 bp, + strand Genetic Position: Chr14, 27.8 cM
• Alliance: Rem2^{tm1c(EUCOMM)Hmgu} page
• IMPC: Rem2 gene page

Mutation origin

• Mutant Cell Line: HEPD0760_4_D07
• Germline Transmission: Earliest citation of germline transmission: J:263600
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 54714536 of Chromosome 14 upstream of the critical exon 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 and 3 at position 54715728. The critical exon 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:263600)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rem2 Mutation: 18 strains or lines available

References

• Original: J:263600 Moore AR, et al., Rem2 stabilizes intrinsic excitability and spontaneous firing in visual circuits. Elife. 2018 May 29;7:e33092
• All: 1 reference(s)