Gt(ROSA)26Sor^{tm1(CAG-EGFP)Brsy}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-EGFP)Brsy}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Kivanc Birsoy
• MGI ID: MGI:6192094
• Synonyms: MITO-Tag, Rosa26^{LSL-3XHA-EGFP-OMP25}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-EGFP)Brsy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268121
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct containing (from 5' t 3') CAG-loxP-neomycin (reversed)-pA-loxP-3xHA-EGFP-SYNJ2BP-lacZ-BGH-pA is targeted to intron 1 of the gene. The 3xHA-EGFP-SYNJ2BP components constitute the "MITO-Tag" cassette targeting the 3XHA epitope tag with GFP to the mitochondrial membrane. The construct includes only the transmembrane domain of the rat Synj2bp/OMP25 (AA109-145) gene. The small fragment of lacZ incorporated in the mutation is not expected to be functional. (J:268121)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:268121 Bayraktar EC, et al., MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo. Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):303-312
• All: 3 reference(s)