Esr1^tm5.1Ksk
Targeted Allele Detail

Summary

• Symbol: Esr1^tm5.1Ksk
• Name: estrogen receptor 1 (alpha); targeted mutation 5.1, Kenneth S Korach
• MGI ID: MGI:6192684
• Synonyms: H2NES
• Gene: Esr1 Location: Chr10:4561989-4955633 bp, + strand Genetic Position: Chr10, 2.03 cM
• Alliance: Esr1^tm5.1Ksk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:263739
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Altered localization)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector is designed to insert arginine to alanine mutations at amino acids 260, 263, 264, 267 and 275, lysine to alanine mutations at amino acids 261, 270 and 272, arginine to leucine at residue 273, and aspartic acid to leucine at residue 276 of the gene. An FRT-flanked neomycin resistance (neo) cassette is inserted in exon 6. Flp-mediated recombination removed the FRT-flanked neo cassette. The mutated region disrupts the nuclear localization sequence of the D domain, and also inserts a nuclear export sequence. (J:263759)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Esr1 Mutation: 68 strains or lines available

References

• Original: J:263739 Stefkovich ML, et al., Experimental models for evaluating non-genomic estrogen signaling. Steroids. 2018 May;133:34-37
• All: 3 reference(s)