Tg(RNU6-1-RNAi:Cdk5)#Chad
Transgene Detail

Summary

• Symbol: Tg(RNU6-1-RNAi:Cdk5)#Chad
• Name: transgene insertion, Changsheng Dong
• MGI ID: MGI:6193754
• Synonyms: Cdk5 knockdown
• Transgene: Tg(RNU6-1-RNAi:Cdk5)#Chad Location: unknown
• Alliance: Tg(RNU6-1-RNAi:Cdk5)#Chad page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Knockdown)
• Mutation: Insertion
• Tg(RNU6-1-RNAi:Cdk5)#Chad involves 1 genes/genome features (Cdk5) View all
• Mutation details: The human U6 small nuclear RNA gene promoter was used to drive expression of a mouse anti-Cdk5 shRNA. The construct was microinjected into the male pronuclei of C57BL/6J zygotes. Three founders were generated. Immunohistochemical and Western blot analyses confirmed that protein expression was decreased in the skin of knockdown mice relative to wild-type controls. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:255707)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:255707 Dong C, et al., Functional Role of Cyclin-Dependent Kinase 5 in the Regulation of Melanogenesis and Epidermal Structure. Sci Rep. 2017 Oct 23;7(1):13783
• All: 1 reference(s)