Tg(Msx2-cre/ERT2)1Nono
Transgene Detail

Summary

• Symbol: Tg(Msx2-cre/ERT2)1Nono
• Name: transgene insertion 1, Noriaki Ono
• MGI ID: MGI:6194241
• Transgene: Tg(Msx2-cre/ERT2)1Nono Location: unknown
• Alliance: Tg(Msx2-cre/ERT2)1Nono page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Msx2-cre/ERT2)1Nono expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Msx2 (MGI:97169)

• Mutation details: The approximately 180kb RP23-438B5 mouse bacterial artificial chromosome (BAC) containing the entire mouse Msx2 gene (and 139kb upstream and 32 kb downstream flanking sequences) was modified by inserting a cre/ERT2 coding sequence, a FRT site flanked neo, and a SV40 polyadenylation signal sequence, into the first exon of the Msx2 gene, replacing the initiation ATG codon. The FRT site flanked neo was removed by transient FLP recombinase induction. DNA from a correctly modified BAC clone was used as the source for the Msx2-creER-SV40pA transgene was microinjected into the pronucleus of fertilized oocytes from hybrid SJL X C57BL/6 mice. Two independent founder lines 1 and 2 were established, and line 2 expressing higher cre/ER gene was used for analyses. (J:263826)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Msx2 (mouse) Summary of all recombinase alleles driven by Msx2. Comparative matrix view of recombinase activities Alliance mouse Msx2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:263826 Sakagami N, et al., Msx2 Marks Spatially Restricted Populations of Mesenchymal Precursors. J Dent Res. 2018 May 1;:22034518771014
• All: 1 reference(s)