Fbxo2^{tm1.1(cre/ERT2)Hels}
Targeted Allele Detail

Summary

• Symbol: Fbxo2^{tm1.1(cre/ERT2)Hels}
• Name: F-box protein 2; targeted mutation 1.1, Stefan Heller
• MGI ID: MGI:6194328
• Gene: Fbxo2 Location: Chr4:148245078-148250881 bp, + strand Genetic Position: Chr4, 78.68 cM
• Alliance: Fbxo2^{tm1.1(cre/ERT2)Hels} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266597
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Fbxo2^{tm1.1(cre/ERT2)Hels} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Fbxo2 (MGI:2446216)

• Mutation details: The targeting vector was designed to insert a multicistronic reporter into the ATG start codon of the F-box protein 2 (Fbxo2) gene. Specifically, this vector includes (from 5' to 3') an H2B-Venus fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] with a C-terminal yellow fluorescent protein sequence), a hygromycin resistance gene flanked with viral P2A oligopeptides, a cre/ERT2 fusion gene (Cre recombinase fused to a human estrogen receptor ligand binding domain), a polyadenylation signal, and a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129P2/OlaHsd-derived E14tg2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with Tg(EIIa-cre)C5379Lmgd mice on an FVB genetic background to remove the neo cassette. (J:266597)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Fbxo2 (mouse) Summary of all recombinase alleles driven by Fbxo2. Alliance mouse Fbxo2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fbxo2 Mutation: 21 strains or lines available

References

• Original: J:266597 Hartman BH, et al., Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination. Dev Biol. 2018 Nov 1;443(1):64-77
• All: 1 reference(s)