Gt(ROSA)26Sor^{tm1(CAG-ECFP*/Rapgef3/Venus*)Kama}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-ECFP*/Rapgef3/Venus*)Kama}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Kirill Martemyanov
• MGI ID: MGI:6194564
• Synonyms: CAMPER
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-ECFP*/Rapgef3/Venus*)Kama} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:263913
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector contains from (5' to 3'): CAG promoter, a loxP STOP loxP cassette, Epac cAMP sensor, WPRE, polyadenylation signal sequence and is inserted between exons 1 and 2 of the Gt(ROSA)26Sor Ai9 targeting vector. The Epac sensor reports cAMP binding by changes in fluorescence energy transfer (FRET). The sensor consists of an mTurqoise donor, a monomeric variant of the cyan fluorescent protein, and a circularly permutated Venus enhanced yellow fluorescent protein, Venus-Venus, acceptor sandwiching the cAMP-binding protein Rapgef3. (J:263913)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:263913 Muntean BS, et al., Interrogating the Spatiotemporal Landscape of Neuromodulatory GPCR Signaling by Real-Time Imaging of cAMP in Intact Neurons and Circuits. Cell Rep. 2018 Jan 2;22(1):255-268
• All: 14 reference(s)