Spata25^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Spata25^{tm1b(EUCOMM)Hmgu}
• Name: spermatogenesis associated 25; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6194645
• Gene: Spata25 Location: Chr2:164669302-164670454 bp, - strand Genetic Position: Chr2, 85.27 cM, cytoband H3
• Alliance: Spata25^{tm1b(EUCOMM)Hmgu} page
• IMPC: Spata25 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234235
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 164669246 of Chromosome 2 (Build GRCm39) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 164669851. The critical artificial exon(s) is/are thus flanked by loxP sites. Cre mediated recombination without flp expression produced a reporter knockout mouse. Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors. (J:234235)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spata25 Mutation: 10 strains or lines available

References

• Original: J:234235 Miyata H, et al., Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice. Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7704-10
• All: 1 reference(s)