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Piezo2em1(IMPC)J
Endonuclease-mediated Allele Detail
Summary
Symbol: Piezo2em1(IMPC)J
Name: piezo-type mechanosensitive ion channel component 2; endonuclease-mediated mutation 1, Jackson
MGI ID: MGI:6195008
Gene: Piezo2  Location: Chr18:63143284-63520254 bp, - strand  Genetic Position: Chr18, 35.95 cM, cytoband D3
Alliance: Piezo2em1(IMPC)J page
IMPC: Piezo2 gene page
Mutation
origin
Strain of Origin:  C57BL/6NJ
Project Collection: IMPC
Mutation
description
Allele Type:    Endonuclease-mediated (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThis allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 4 guide sequences TGGGGCTCATTTCAACCTAA, CCTTGACGTTGGAAAGCCAA, TTACTACACACTAGGTTATG and GCAAACGTCTCCACTCTCCT, which resulted in a 443 bp deletion beginning at Chromosome 18 position 63,030,248 bp and ending after 63,030,690 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001304790 (exon 42) and 190 bp of flanking intronic sequence including the splice acceptor and donor. At the deletion site there is a 126 bp insertion (AGAATGGGGACGAGGAGTGTTAGGATATTAATAAAGAACACTATTAGGGAGAGGATTTGAACCTCTGGGAACAAGGTTTTAAGTCTTACGCAATTTCCTGGCTCTGCCACCCTAATAACCTTCTCT) that is from the mouse mitochondrial genome (ChrM 2666-2791). This 126 bp segment contains the coding sequence for mt-Tl1 (tRNA leucine 1). The exon deletion is predicted to cause a change of amino acid sequence after residue 2106 and early truncation 15 amino acids later. (J:188991)
Inheritance:    Not Specified
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Piezo2 Mutation:  123 strains or lines available
References
Original:  J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory