Lyz2^{em1.1(cre)Elgaz}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Lyz2^{em1.1(cre)Elgaz}
• Name: lysozyme 2; endonuclease-mediated mutation 1.1, Mohamed Elgazzar
• MGI ID: MGI:6195189
• Synonyms: Lyz2-Cre, Lyz2^em1.1Elgaz
• Gene: Lyz2 Location: Chr10:117113446-117118177 bp, - strand Genetic Position: Chr10, 65.34 cM
• Alliance: Lyz2^{em1.1(cre)Elgaz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244204
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: BALB/cJ

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Lyz2^{em1.1(cre)Elgaz} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lyz2 (MGI:96897)

• Mutation details: CRISPR/cas9 endonuclease mediated genome editing is used to introduce a nucleus-localized (NLS) Cre recombinase followed by a FRT-flanked neomycin cassette into the ATG start codon of the gene. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:244204)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lyz2 (mouse) Summary of all recombinase alleles driven by Lyz2. Alliance mouse Lyz2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lyz2 Mutation: 40 strains or lines available

Notes

ES Cell Line = PRX-BALB/cJ 9

References

• Original: J:244204 McPeak MB, et al., Myeloid Cell-Specific Knockout of NFI-A Improves Sepsis Survival. Infect Immun. 2017 Apr;85(4)
• All: 3 reference(s)