Arc^tm2.1Grnt
Targeted Allele Detail

Summary

• Symbol: Arc^tm2.1Grnt
• Name: activity regulated cytoskeletal-associated protein; targeted mutation 2.1, Seth G N Grant
• MGI ID: MGI:6195289
• Synonyms: Arc^Venus
• Gene: Arc Location: Chr15:74540932-74544419 bp, - strand Genetic Position: Chr15, 34.25 cM
• Alliance: Arc^tm2.1Grnt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:254217
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: Sequence coding for a TAP (Tandem Affinity Purification) tag (containing Histidine Affinity Tag (HAT), TEV protease and 3x Flag sequences), a linker (coding for GGGS), and the Venus yellow fluorescent reporter, followed by a loxP site flanked neomycin resistance gene cassette, was inserted before the translation stop codon in exon 1. The neo cassette was removed through subsequent cre-mediated recombination. This leaves an allele coding for a chimeric protein of the endogenous protein with a C-terminal TAP tag and Venus peptide. (J:254217)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Arc Mutation: 33 strains or lines available

References

• Original: J:254217 Fernandez E, et al., Arc Requires PSD95 for Assembly into Postsynaptic Complexes Involved with Neural Dysfunction and Intelligence. Cell Rep. 2017 Oct 17;21(3):679-691
• All: 1 reference(s)