Abcc6^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Abcc6^{tm1c(EUCOMM)Wtsi}
• Name: ATP-binding cassette, sub-family C member 6; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6195711
• Synonyms: Abcc6^flox
• Gene: Abcc6 Location: Chr7:45625804-45679915 bp, - strand Genetic Position: Chr7, 29.64 cM, cytoband B3
• Alliance: Abcc6^{tm1c(EUCOMM)Wtsi} page
• IMPC: Abcc6 gene page

Mutation origin

• Mutant Cell Line: EPD0656_4_F08
• Germline Transmission: Earliest citation of germline transmission: J:252837
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 46019130 of Chromosome 7 upstream of exon 5. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 46017355. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 5 and 6 floxed. (J:252837)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Abcc6 Mutation: 76 strains or lines available

References

• Original: J:252837 Ziegler SG, et al., Ectopic calcification in pseudoxanthoma elasticum responds to inhibition of tissue-nonspecific alkaline phosphatase. Sci Transl Med. 2017 Jun 07;9(393)
• All: 1 reference(s)